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- aggregation classification "A1".
- aggregation creator B256188.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
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- aggregation date "2010".
- aggregation format "application/pdf".
- aggregation hasFormat 1106346.bibtex.
- aggregation hasFormat 1106346.csv.
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- aggregation hasFormat 1106346.doc.
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- aggregation isPartOf urn:issn:1110-7243.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Dynamic metabolic flux analysis demonstrated on cultures where the limiting substrate is changed from carbon to nitrogen and vice versa".
- aggregation abstract "The main requirement for metabolic flux analysis (MFA) is that the cells are in a pseudo-steady state, that there is no accumulation or depletion of intracellular metabolites. In the past, the applications of MFA were limited to the analysis of continuous cultures. This contribution introduces the concept of dynamic MFA and extends MFA so that it is applicable to transient cultures. Time series of concentration measurements are transformed into flux values. This transformation involves differentiation, which typically increases the noisiness of the data. Therefore, a noise-reducing step is needed. In this work, polynomial smoothing was used. As a test case, dynamic MFA is applied on Escherichia coli cultivations shifting from carbon limitation to nitrogen limitation and vice versa. After switching the limiting substrate from N to C, a lag phase was observed accompanied with an increase in maintenance energy requirement. This lag phase did not occur in the C-to N-limitation case.".
- aggregation authorList BK530680.
- aggregation aggregates 1108813.
- aggregation isDescribedBy 1106346.
- aggregation similarTo 621645.
- aggregation similarTo LU-1106346.