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- aggregation classification "A1".
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "1999".
- aggregation format "application/pdf".
- aggregation hasFormat 114703.bibtex.
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- aggregation isPartOf urn:issn:0007-1048.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Bone marrow CD34+ cells generate fewer T cells in vitro with increasing age and following chemotherapy".
- aggregation abstract "To assess the influence of high-dose chemotherapy and age on the intrinsic capacity of stem cells to generate T cells, CD34(+) cells derived from bone marrow used in clinical transplantation were evaluated in an in vitro T-cell assay using a mouse thymic microenvironment. CD34(+) cells were sorted from healthy donor and autologous back-up bone marrow after density gradient centrifugation and depletion for CD1, 3, 4, 7, 8, 19 and glycophorin A using magnetic beads. CD34(+) cells were then introduced in day 14-15 fetal SCID mouse thymus lobes by incubation in hanging drops for 48 h. After transfer to gelfoam rafts they were cultured for variable time periods, The robes were then homogenized in a tissue grinder for now cytometric analysis gating on human cells. These were evaluated for CD4, CD8, CD3 and HLA-DR surface expression 51 samples were analysed and three patterns of T-cell precursor development could be observed. In pattern A no human cells could be recovered, in pattern B maturation stopped at the CD4(+)CD8(-)CD3(-) pre-T-cell stage, and in pattern C maturation to double-positive CD4(+)CD8(+) thymocytes was reached. In 25 healthy donors under age 40 three showed pattern A, 12 pattern B and 10 pattern C, whereas in 16 healthy donors over the age 40 there were respectively four with A, seven with B and only five with C (P=0.01), In 10 patients who had previously received chemotherapy, none developed pattern C, five pattern B and five pattern A, in contrast to 15/41 pattern C, 19/41 pattern B and 7/41 pattern A in healthy donors. These data suggest an intrinsic loss of T-cell generation capacity from adult bone marrow stem cells in comparison to reports on stem cells of fetal origin. This loss correlated weakly with age, irrespective of thymic involution, and may be further reduced by prior chemotherapy.".
- aggregation authorList BK73564.
- aggregation endPage "808".
- aggregation issue "4".
- aggregation startPage "801".
- aggregation volume "104".
- aggregation aggregates 2030807.
- aggregation isDescribedBy 114703.
- aggregation similarTo j.1365-2141.1999.01265.x.
- aggregation similarTo LU-114703.