Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1151765#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B607504.
- aggregation creator person.
- aggregation creator person.
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- aggregation date "2010".
- aggregation format "application/pdf".
- aggregation hasFormat 1151765.bibtex.
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- aggregation hasFormat 1151765.doc.
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- aggregation isPartOf urn:issn:0022-1317.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Veterinary Sciences".
- aggregation title "Replication kinetics of neurovirulent versus non-neurovirulent equine herpesvirus type 1 strains in equine nasal mucosal explants".
- aggregation abstract "Equine herpesvirus type 1 (EHV-1) is the causative agent of equine herpes myeloencephalopathy, of which outbreaks are reported with increasing frequency throughout North America and Europe. This has resulted in its classification as a potentially emerging disease by the US Department of Agriculture. Recently, it was found that a single nucleotide polymorphism (SNP) in the viral DNA polymerase gene (ORF30) at aa 752 (N -> D) is associated with the neurovirulent potential of EHV-1. In the present study, equine respiratory mucosal explants were inoculated with several Belgian isolates typed in their ORF30 as D-752 or N-752, to evaluate a possible difference in replication in the upper respiratory tract. In addition, to evaluate whether any observed differences could be attributed to the SNP associated with neurovirulence, the experiments were repeated with parental Ab4 (reference neurovirulent strain), parental NY03 (reference non-neurovirulent strain) and their N/D revertant recombinant viruses. The salient findings were that EHV-1 spreads plaquewise in the epithelium, but plaques never cross the basement membrane (BM). However, single EHV-1-infected cells could be observed below the BM at 36 h post-inoculation (p.i.) for all N-752 isolates and at 24 h p.i. for all D-752 isolates, and were identified as monocytic cells and T lymphocytes. Interestingly, the number of infected cells was two to five times higher for D-752 isolates compared with N-752 isolates at every time point analysed. Finally, this study showed that equine respiratory explants are a valuable and reproducible model to study EHV-1 neurovirulence in vitro, thereby reducing the need for horses as experimental animals.".
- aggregation authorList BK965417.
- aggregation endPage "2028".
- aggregation issue "8".
- aggregation startPage "2019".
- aggregation volume "91".
- aggregation aggregates 1151787.
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- aggregation similarTo vir.0.019257-0.
- aggregation similarTo LU-1151765.