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- aggregation classification "A1".
- aggregation creator B607399.
- aggregation creator B607400.
- aggregation creator person.
- aggregation date "2007".
- aggregation format "application/pdf".
- aggregation hasFormat 1203439.bibtex.
- aggregation hasFormat 1203439.csv.
- aggregation hasFormat 1203439.dc.
- aggregation hasFormat 1203439.didl.
- aggregation hasFormat 1203439.doc.
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- aggregation hasFormat 1203439.yaml.
- aggregation isPartOf urn:issn:0959-6658.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "P-selectin mediates metastatic progression through binding to sulfatides on tumor cells".
- aggregation abstract "Hematogenous carcinoma metastasis is associated with tumor cell emboli formation, which is now known to be facilitated by selectins. P-selectin-mediated interactions of platelets with cancer cells are based mostly on mucin- and glycosaminoglycan-type selectin ligands. We previously showed that mouse colon carcinoma cells (MC-38) carry P-selectin ligands of nonmucin origin, which were not identified. Here we show that P-selectin ligands recognized on MC-38 cells are sulfated glycolipids, thereby facilitating experimental metastasis in a syngeneic mouse model. Metabolic inhibition of sulfation by incubation of cells with sodium chlorate almost completely abrogated P-selectin binding. Metabolic labeling of MC-38 cells with S-35 sulfate revealed only a single band as detected by high-performance thin layer chromatography analysis of a total lipid extract. Matrix-assisted laser desorption/ionization tandem time-of-flight/time-of-flight analysis (MALDI-TOF-TOF) analysis of the purified sulfate-containing lipid fraction identified the selectin ligand to be a sulfated galactosylceramide SM4 (HSO3-3Gal beta-1Cer). Modulation of glycolipid biosynthesis in MC-38 cells altered P-selectin binding, thereby confirming sulfoglycolipids to be major P-selectin ligands. In addition, P-selectin was also found to recognize lactosylceramide sulfate SM3 (HSO3-3Gal beta-4Glc beta-1Cer) and gangliotriaosylceramide sulfate SM2 [GalNAc beta-4(HSO3-3)Gal beta-4Glc beta-1Cer] in human hepatoma cells. Finally, the enzymatic removal of sulfation from the cell surface of MC-38 cells resulted in decreased P-selectin binding and led to attenuation of metastasis. Thus, SM4 sulfatide serves as a native ligand for P-selectin contributing to cell-cell interactions and to facilitation of metastasis.".
- aggregation authorList BK965183.
- aggregation endPage "196".
- aggregation issue "2".
- aggregation startPage "185".
- aggregation volume "17".
- aggregation aggregates 1203445.
- aggregation isDescribedBy 1203439.
- aggregation similarTo cw1059.
- aggregation similarTo LU-1203439.