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- aggregation classification "A1".
- aggregation creator person.
- aggregation creator person.
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- aggregation creator person.
- aggregation date "2011".
- aggregation format "application/pdf".
- aggregation hasFormat 1209010.bibtex.
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- aggregation isPartOf urn:issn:1618-0240.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Science General".
- aggregation title "A constitutive expression system for high-throughput screening".
- aggregation abstract "To reduce the amount of consumables and number of pipetting steps in high-throughput screening, a constitutive expression system was developed that comprises four different promoters of varying strength. The system was validated by the expression of different sucrose phosphorylase enzymes from Leuconostoc mesenteroides, Lactobacillus acidophilus and Bifidobacterium adolescentis in 96-deep-and low-well plates at three temperatures. Drastically improved soluble expression in mini-cultures was observed for the enzymes from L. mesenteroides strains by reducing the promoter strength from strong to intermediate and by expressing the proteins at lower temperatures. In contrast, the enzymes from B. adolescentis and L. acidophilus were expressed most efficiently with a strong promoter. The constitutive expression of sucrose phosphorylases in low-well plates resulted in a level of activity that is equal or even better than what was achieved by inducible expression. Therefore, our plasmid set with varying constitutive promoters will be an indispensable tool to optimize enzyme expression for high-throughput screening.".
- aggregation authorList BK691979.
- aggregation endPage "19".
- aggregation issue "1".
- aggregation startPage "10".
- aggregation volume "11".
- aggregation aggregates 3148053.
- aggregation isDescribedBy 1209010.
- aggregation similarTo elsc.201000065.
- aggregation similarTo LU-1209010.