Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1227832#aggregation> ?p ?o. }
Showing items 1 to 38 of
38
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B290968.
- aggregation creator B290969.
- aggregation creator B290970.
- aggregation creator B290971.
- aggregation creator B290972.
- aggregation creator B290973.
- aggregation creator person.
- aggregation date "2002".
- aggregation format "application/pdf".
- aggregation hasFormat 1227832.bibtex.
- aggregation hasFormat 1227832.csv.
- aggregation hasFormat 1227832.dc.
- aggregation hasFormat 1227832.didl.
- aggregation hasFormat 1227832.doc.
- aggregation hasFormat 1227832.json.
- aggregation hasFormat 1227832.mets.
- aggregation hasFormat 1227832.mods.
- aggregation hasFormat 1227832.rdf.
- aggregation hasFormat 1227832.ris.
- aggregation hasFormat 1227832.txt.
- aggregation hasFormat 1227832.xls.
- aggregation hasFormat 1227832.yaml.
- aggregation isPartOf urn:issn:0929-1903.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Efficient genetic modification of murine dendritic cells by electroporation with mRNA".
- aggregation abstract "Recently, human dendritic cells (DCs) pulsed with mRNA encoding a broad range of tumor antigens have proven to be potent activators of a primary anti-tumor-specific T-cell response in vitro. The aim of this study was to improve the mRNA pulsing of murine DC. Compared to a standard lipofection protocol and passive pulsing, electroporation was, in our hands, the most efficient method. The optimal conditions to electroporate murine bone marrow-derived DCs with mRNA were determined using enhanced green fluorescent protein and a truncated form of the nerve growth factor receptor. We could obtain high transfection efficiencies around 70-80% with a mean fluorescence intensity of 100-200. A maximal expression level was reached 3 hours after electroporation. A clear dose-response effect was seen depending on the amount of mRNA used. Importantly, the electroporation process did not affect the viability nor the allostimulatory capacity or phenotype of the DC. To study the capacity of mRNA-electroporated DCs to present antigen in the context of MHC classes I and II, we made use of chimeric constructs of ovalbumin. The dose-dependent response effect and the duration of presentation were also determined. Together, these results demonstrate that mRNA electroporation is a useful method to generate genetically modified murine DC, which can be used for preclinical studies testing immunotherapeutic approaches.".
- aggregation authorList BK574719.
- aggregation endPage "797".
- aggregation issue "9".
- aggregation startPage "787".
- aggregation volume "9".
- aggregation aggregates 1263240.
- aggregation isDescribedBy 1227832.
- aggregation similarTo sj.cgt.7700499.
- aggregation similarTo LU-1227832.