Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1227874#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B585105.
- aggregation creator B585106.
- aggregation creator B585107.
- aggregation creator B585108.
- aggregation creator B585109.
- aggregation creator person.
- aggregation date "2005".
- aggregation format "application/pdf".
- aggregation hasFormat 1227874.bibtex.
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- aggregation hasFormat 1227874.doc.
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- aggregation isPartOf urn:issn:1525-0016.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "In vivo depletion of CD4⁺CD25⁺ regulatory T cells enhances the antigen-specific primary and memory CTL response elicited by mature mRNA-electroporated dendritic cells".
- aggregation abstract "We previously described mRNA electroporation as an efficient gene delivery method to introduce tumor-antigens (Ag) into murine immature dendritic cells (DC). Here, we further optimize the protocol and evaluate the capacity of mRNA-electroporated DC as a vaccine for immunotherapy. First, the early DC maturation kinetics and the effect of different lipopolysaccha ride incubation periods on the phenotypic maturation profile of DC are determined. Next, we show that either immature or mature DC are equally well electroporated and express and present the transgene at a comparable level after electroporation. We point out that the mRNA electroporation results in a negative effect on the interleukin (IL)-12p70, IL-6, and tumor necrosis factor-alpha secretion after maturation. Nevertheless, mRNA-electroporated DC induce an effective cytotoxic T lymphocyte (CTL) response in vivo. Mature electroporated DC are significantly more potent in eliciting an Ag-specific CD8(+) CTL response compared to their immature electroporated counterparts. In addition, a significant improvement in CTL response is obtained both in the primary and in the memory effector phases when CD4(+)CD25(+) regulatory T cells (Treg) are depleted in vivo prior to immunization. These findings are further substantiated in tumor protection experiments and hold convincing evidence for the merit of Treg cell depletion prior to immunization with mRNA-electroporated DC.".
- aggregation authorList BK938633.
- aggregation endPage "932".
- aggregation issue "5".
- aggregation startPage "922".
- aggregation volume "12".
- aggregation aggregates 3118676.
- aggregation isDescribedBy 1227874.
- aggregation similarTo j.ymthe.2005.02.029.
- aggregation similarTo LU-1227874.