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- aggregation classification "A1".
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2000".
- aggregation format "application/pdf".
- aggregation hasFormat 125033.bibtex.
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- aggregation isPartOf urn:issn:0026-8925.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism".
- aggregation abstract "Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII) transgenes, whereas HOlo2 plants, homozygous for a single T-DNA insert, expressed the nptII genes normally. Transient expression of nptII genes newly introduced into leaves of both the HOlo2 and nptII-silenced HOlo1 plants was downregulated only in the silenced background. Different chimeric beta-glucuronidase (gus) genes with parts of the nptII transgene inserted in sense or antisense orientation into the 3'-untranslated region, which encoded transcripts that had homology or complementarity to nptII transcripts, showed reduced transient expression specifically in npII-silenced tissue. Therefore, we conclude that RNAs of both polarities are targets for PTGS-induced RNA degradation, which supports the notion that double-stranded RNA acts as an inducing signal for silencing.".
- aggregation authorList BK260507.
- aggregation endPage "1002".
- aggregation issue "6".
- aggregation startPage "995".
- aggregation volume "263".
- aggregation aggregates 4151075.
- aggregation isDescribedBy 125033.
- aggregation similarTo PL00008700.
- aggregation similarTo LU-125033.