Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1254197#aggregation> ?p ?o. }
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- aggregation classification "C3".
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2010".
- aggregation hasFormat 1254197.bibtex.
- aggregation hasFormat 1254197.csv.
- aggregation hasFormat 1254197.dc.
- aggregation hasFormat 1254197.didl.
- aggregation hasFormat 1254197.doc.
- aggregation hasFormat 1254197.json.
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- aggregation hasFormat 1254197.mods.
- aggregation hasFormat 1254197.rdf.
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- aggregation hasFormat 1254197.txt.
- aggregation hasFormat 1254197.xls.
- aggregation hasFormat 1254197.yaml.
- aggregation language "eng".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Efficient large scale expansion of human embryonic stem cells: comparison of 4 different solutions for single cell passaging".
- aggregation abstract "Human embryonic stem cells (hESC) are promising for tissue engineering (TE) purposes due to their unique properties. However, the current standard mechanical passaging technique limits the rate of possible TE experiments. Alternative single cell passaging techniques have been described in literature with varying results. In this comparative study, the H1 and VUB02 hESC lines were tested in combination with a fast and slow adaptation protocol and 4 different dissociative solutions to determine the best strategy to quickly obtain large amounts of stable hESC. The following dissociative solutions were examined: TrypLE™ Express, Trypsin-EDTA, Cell Dissociation Solution and Accutase™. Results showed that it was possible to generate pluripotent hESC colonies using all 4 dissociative solutions. The use of Cell Dissociation Solution proved to be the most efficient, as it generated the most colonies and sustained a high and stable expansion rate for both hESC cell lines. Application of the slow adaptation protocol resulted in an overall higher and quicker expansion rate than when the fast adaptation protocol was applied. Additionally, Cell Dissociation Solution is an enzyme- and animal-free solution, which makes these results more interesting regarding future clinical applications. The obtained hESC cell lines maintained their pluripotent capacity and had a stable karyotypic profile. We conclude that Cell dissociation solution in combination with a slow adaptation protocol allows a fast switch from the traditional mechanical passaging technique to a single cells split technique, generating a stable and robust hESC cell line, which allows large scale expansion for TE purposes.".
- aggregation authorList BK303490.
- aggregation isDescribedBy 1254197.
- aggregation similarTo LU-1254197.