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- aggregation classification "A1".
- aggregation creator person.
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- aggregation date "1997".
- aggregation format "application/pdf".
- aggregation hasFormat 182813.bibtex.
- aggregation hasFormat 182813.csv.
- aggregation hasFormat 182813.dc.
- aggregation hasFormat 182813.didl.
- aggregation hasFormat 182813.doc.
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- aggregation isPartOf urn:issn:0735-9640.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Detection of transcripts via fluorescence in-situ hybridization and confocal microscopy in whole mounts of roots of Arabidopsis thaliana".
- aggregation abstract "We report a modification of a previously published procedure (de Almeida Engler et al., 1994) aimed at the fluorescent detection of RNA-RNA hybrids. Transcripts of ARAth;Cyc1;1 were located in whole mounts of roots of Arabidopsis thaliana after in-situ hybridization with digoxigenin- and biotin-labeled antisense probes. Digoxigenin- and biotin-labeled hybrids were detected by FISH with an AP-conjugated antibody against digoxigenin or AP-conjugated streptavidin for detection of biotin, with the UV-excitable dye ELF(TM) as a substrate. Alternatively, biotin-labeled hybrids were detected with HRP-conjugated streptavidin, with fluorescein tyramine as a substrate. Images were recorded via confocal microscopy. We report on the different fixation and reaction conditions needed for obtaining the highest signal-to-noise ratio, as well as on the requirements for mounting media, filter sets, excitation wavelengths, and recording modes used for recording confocal data sets.".
- aggregation authorList BK335607.
- aggregation endPage "37".
- aggregation issue "1".
- aggregation startPage "22".
- aggregation volume "15".
- aggregation aggregates 1907984.
- aggregation isDescribedBy 182813.
- aggregation similarTo BF02772110.
- aggregation similarTo LU-182813.