Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1888421#aggregation> ?p ?o. }
Showing items 1 to 36 of
36
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B410405.
- aggregation creator B410406.
- aggregation creator B410407.
- aggregation creator B410408.
- aggregation creator person.
- aggregation date "2011".
- aggregation format "application/pdf".
- aggregation hasFormat 1888421.bibtex.
- aggregation hasFormat 1888421.csv.
- aggregation hasFormat 1888421.dc.
- aggregation hasFormat 1888421.didl.
- aggregation hasFormat 1888421.doc.
- aggregation hasFormat 1888421.json.
- aggregation hasFormat 1888421.mets.
- aggregation hasFormat 1888421.mods.
- aggregation hasFormat 1888421.rdf.
- aggregation hasFormat 1888421.ris.
- aggregation hasFormat 1888421.txt.
- aggregation hasFormat 1888421.xls.
- aggregation hasFormat 1888421.yaml.
- aggregation isPartOf urn:issn:2041-1006.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Live/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies".
- aggregation abstract "DNA-based methodology for the identification and detection of specific bacteria in dental plaque offers advantages over culturing techniques. One drawback of current molecular techniques like real-time quantitative polymerase chain reaction (RT-QPCR) is that they are not able to distinguish between live or dead bacteria. To overcome this problem an assay was assessed to discriminate between viable or dead bacteria using DNA intercalating substances, propidium monoazide (PMA) and ethidium monoazide (EMA) in combination with RT-QPCR. The assay was tested on oral pathogens: Streptococcus mutans, Prevotella intermedia and Aggregatibacter actinomycetemcomitans. To determine the effectiveness of EMA and PMA, different concentrations (from 5 to 100 mu g ml(-1)) of the substances were added to viable or heat-killed suspensions of both organisms (ranging from 10(8) to 10(4) colony-forming units ml(-1)). Afterwards, PMA was tested on mixtures of varying ratios of viable and dead cells. After DNA extraction, RT-QPCR was performed using species-specific primers. Both compounds inhibited PCR amplification from dead cells. The EMA treatment resulted in the largest signal decrease but EMA also inhibited DNA amplification from viable cells. For this reason, PMA was selected for use in further experiments. It was shown to be efficient in allowing selective PCR detection of only viable cells in mixtures containing both viable and dead cells. The amount of amplified DNA corresponded to the percentage of viable cells in the sample. The developed assay will potentially be useful for assessing bacterial loads remaining after disinfection protocols without interference by nonviable bacteria.".
- aggregation authorList BK727101.
- aggregation endPage "261".
- aggregation issue "4".
- aggregation startPage "253".
- aggregation volume "26".
- aggregation aggregates 1888481.
- aggregation isDescribedBy 1888421.
- aggregation similarTo j.2041-1014.2011.00615.x.
- aggregation similarTo LU-1888421.