Matches in UGent Biblio for { <https://biblio.ugent.be/publication/189507#aggregation> ?p ?o. }
Showing items 1 to 35 of
35
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B76216.
- aggregation creator B76217.
- aggregation creator B76218.
- aggregation creator B76219.
- aggregation creator B76220.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "1996".
- aggregation hasFormat 189507.bibtex.
- aggregation hasFormat 189507.csv.
- aggregation hasFormat 189507.dc.
- aggregation hasFormat 189507.didl.
- aggregation hasFormat 189507.doc.
- aggregation hasFormat 189507.json.
- aggregation hasFormat 189507.mets.
- aggregation hasFormat 189507.mods.
- aggregation hasFormat 189507.rdf.
- aggregation hasFormat 189507.ris.
- aggregation hasFormat 189507.txt.
- aggregation hasFormat 189507.xls.
- aggregation hasFormat 189507.yaml.
- aggregation isPartOf urn:issn:0166-0934.
- aggregation language "eng".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Isolation of HIV-1 RNA from plasma: Evaluation of eight different extraction methods.".
- aggregation abstract "The efficacy of eight different methods for the extraction of HIV-1 RNA from plasma was compared. The RNA preparation method that gave the best results by RT-PCR was the one described by Chomczynski and Sacchi (1987, Anal. Biochem. 162, 156-159). This method consists of a guanidine thiocyanate treatment followed by three phenol-chloroform-isoamylalcohol extractions and an ethanol precipitation. The disadvantage of this method is that it is time consuming and less suitable for the extraction of large series of samples. Moreover, due to the large number of procedural steps, there is a greater risk of sample mix-up or contamination. Of the single-step RNA purification methods, good results were obtained with the TRIzol method (Gibco Life Technologies, Paisley, UK) and with the extraction method offered by the NASBA kit (Organon Teknika, Turnhout, Belgium). The above single-step methods are recommended since both are sensitive enough to detect low copy numbers of HIV-RNA in the plasma of asymptomatic patients, and require only 2 h for completion. For most of the methods evaluated the inter-test variablity was acceptable (mean variation coefficient between duplicate extraction varied between 17.3 and 47.3%). Inter-laboratory reproducibility was evaluated only for the TRIzol-method and found to be low (mean variation coefficient 63.4).".
- aggregation authorList BK194306.
- aggregation endPage "159".
- aggregation issue "2".
- aggregation startPage "155".
- aggregation volume "60".
- aggregation isDescribedBy 189507.
- aggregation similarTo LU-189507.