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- aggregation classification "A1".
- aggregation creator B258449.
- aggregation creator B258450.
- aggregation creator B258451.
- aggregation creator B258452.
- aggregation creator B258453.
- aggregation creator B258454.
- aggregation creator B258455.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2011".
- aggregation format "application/pdf".
- aggregation hasFormat 1908812.bibtex.
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- aggregation isPartOf urn:issn:0955-3002.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes".
- aggregation abstract "Purpose: The in vitro micronucleus (MN) assay is a reliable method to assess radiation-induced chromosomal damage in human peripheral blood lymphocytes. It is used to evaluate in vivo radiation over-exposure and to assess in vitro chromosomal radiosensitivity. A limitation of the MN assay is the relatively high and variable spontaneous MN frequency that restricts low-dose estimation to doses of about 0.3 gray (Gy). As radiation-induced MN mainly contain acentric fragments and spontaneous MN originate from lagging chromosomes, both MN types can be distinguished from each other by using fluorescence in situ hybridisation (FISH) with a pan-centromeric probe. The aim of this study was to investigate if the sensitivity, reliability and processing time of the MN assay can be enhanced by combining the automated MN assay with pan-centromere scoring. Materials and methods: Blood samples from 10 healthy donors were irradiated in vitro with low doses of gamma-rays. Dose response curves were determined for fully-automated and semi-automated MN scoring and semi-automated scoring of centromere negative MN (MNCM-). Results: A good correlation was obtained between fully-automated and semi-automated MN scoring (r<SU2</SU == 0.9973) and between fully automated MN scoring and semi-automated scoring of MNCM- (r<SU2</SU == 0.998). With the Wilcoxon test, a significant p value was obtained between 0 and 0.2 Gy for the fully-automated MN analysis, between 0 and 0.1 Gy for semi-automated MN analysis and between 0 and 0.05 Gy for semi-automated scoring of MNCM-. Conclusion: The semi-automated micronucleus-centromere assay combines high-speed MN analysis with a more accurate assessment in the low-dose range which makes it of special interest for large-scale radiation applications.".
- aggregation authorList BK535238.
- aggregation endPage "931".
- aggregation issue "9".
- aggregation startPage "923".
- aggregation volume "87".
- aggregation aggregates 1908845.
- aggregation isDescribedBy 1908812.
- aggregation similarTo 09553002.2011.577508.
- aggregation similarTo LU-1908812.