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- aggregation classification "A1".
- aggregation creator B258528.
- aggregation creator person.
- aggregation creator person.
- aggregation date "1988".
- aggregation format "application/pdf".
- aggregation hasFormat 1918854.bibtex.
- aggregation hasFormat 1918854.csv.
- aggregation hasFormat 1918854.dc.
- aggregation hasFormat 1918854.didl.
- aggregation hasFormat 1918854.doc.
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- aggregation hasFormat 1918854.mods.
- aggregation hasFormat 1918854.rdf.
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- aggregation hasFormat 1918854.yaml.
- aggregation isPartOf urn:issn:0022-2836.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "vir-Induced recombination in Agrobacterium: physical characterization of precise and imprecise T-circle formation".
- aggregation abstract "Induction of Ti plasmid virulence (vir) gene expression during the early stages of plant cell transformation by Agrobacterium tumefaciens initiates the generation of several T-DNA-associated molecular events: (1) site-specific nicks at T-DNA border sequences (border nicks); (2) free, unipolar, linear, single-stranded T-DNA copies (T-strands); and (3) double-stranded, circular T-DNA molecules (T-circles). The first two T-DNA products have been detected in A. tumefaciens, while T-circles have only been detected following Escherichia coli transformation or transduction. The relationship between the three events has not been evaluated since the genesis of T-circles in A. tumefaciens has not been clarified. Evidence is presented here that T-circles are not an artefact of E. coli transformation, but are present as free, double-stranded molecules in A. tumefaciens resulting from site-specific reciprocal recombination between the left and right 25-base-pair border sequences that flank the T-DNA. Furthermore, the frequency of T-circle formation correlates with the frequency of formation of its reciprocal product, the Ti plasmid deleted in the T-DNA region. Several types of recombinant T-DNA circles arise after activation of vir gene expression, a major class representing precise site-specific recombination between both T-DNA borders, and a minor class representing recombination events either utilizing only one T-DNA border sequence and other Ti plasmid sequences, or utilizing only Ti plasmid sequences (i.e. no T-DNA borders). Nucleotide sequence analyses show that when one (nicked) border recombines with other Ti plasmid sequences, a small stretch (16 to 17 base-pairs) of local homology suffices to allow crossing over.".
- aggregation authorList BK535361.
- aggregation endPage "384".
- aggregation issue "2".
- aggregation startPage "373".
- aggregation volume "203".
- aggregation aggregates 1962953.
- aggregation isDescribedBy 1918854.
- aggregation similarTo 0022-2836(88)90005-8.
- aggregation similarTo LU-1918854.