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- aggregation classification "A1".
- aggregation creator person.
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- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 1997615.bibtex.
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- aggregation isPartOf urn:issn:1871-6784.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Technology and Engineering".
- aggregation title "Increasing recombinant protein production in Escherichia coli K12 through metabolic engineering".
- aggregation abstract "Escherichia coli strains are widely used as host for the production of recombinant proteins. Compared to E. coli K12, E. coli BL21 (DE3) has several biotechnological advantages, such as a lower acetate yield and a higher biomass yield, which have a beneficial effect on protein production. In a previous study (BMC Microbiol. 2011, 11:70) we have altered the metabolic fluxes of a K12 strain (i.e. E. coli MG1655) by deleting the regulators ArcA and IclR in such a way that the biomass yield is remarkably increased, while the acetate production is decreased to a similar value as for BL21 (DE3). In this study we show that the increased biomass yield beneficially influences recombinant protein production as a higher GFP yield was observed for the double knockout strain compared to its wild type. However, at higher cell densities (>2 g LÀ1 CDW), the GFP concentration decreases again, due to the activity of proteases which obstructs the application of the strain in high cell density cultivations. By further deleting the genes lon and ompT, which encode for proteases, this degradation could be reduced. Consequently, higher GFP yields were observed in the quadruple knockout strain as opposed to the double knockout strain and the MG1655 wild type and its yield approximates the GFP yield of E. coli BL21 (DE3), that is, 27 +- 5 mg g/g vs. CDW 30 +- 5 mg g/g , respectively.".
- aggregation authorList BK1037695.
- aggregation endPage "261".
- aggregation issue "2".
- aggregation startPage "255".
- aggregation volume "30".
- aggregation aggregates 4409821.
- aggregation isDescribedBy 1997615.
- aggregation similarTo j.nbt.2011.11.008.
- aggregation similarTo LU-1997615.