Matches in UGent Biblio for { <https://biblio.ugent.be/publication/2042335#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B582702.
- aggregation creator B582703.
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- aggregation creator B582705.
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- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 2042335.bibtex.
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- aggregation isPartOf urn:issn:0028-0836.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "A novel sensor to map auxin response and distribution at high spatio-temporal resolution".
- aggregation abstract "Auxin is a key plant morphogenetic signal(1) but tools to analyse dynamically its distribution and signalling during development are still limited. Auxin perception directly triggers the degradation of Aux/IAA repressor proteins(2-6). Here we describe a novel Aux/IAA-based auxin signalling sensor termed DII-VENUS that was engineered in the model plant Arabidopsis thaliana. The VENUS fast maturing form of yellow fluorescent protein(7) was fused in-frame to the Aux/IAA auxin-interaction domain (termed domain II; DII)(5) and expressed under a constitutive promoter. We initially show that DII-VENUS abundance is dependent on auxin, its TIR1/AFBs co-receptors(4-6,8) and proteasome activities. Next, we demonstrate that DII-VENUS provides a map of relative auxin distribution at cellular resolution in different tissues. DII-VENUS is also rapidly degraded in response to auxin and we used it to visualize dynamic changes in cellular auxin distribution successfully during two developmental responses, the root gravitropic response and lateral organ production at the shoot apex. Our results illustrate the value of developing response input sensors such as DII-VENUS to provide high-resolution spatio-temporal information about hormone distribution and response during plant growth and development.".
- aggregation authorList BK934808.
- aggregation endPage "106".
- aggregation issue "7383".
- aggregation startPage "103".
- aggregation volume "482".
- aggregation aggregates 2042535.
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