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- aggregation classification "A1".
- aggregation creator B150352.
- aggregation creator B150353.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "1992".
- aggregation hasFormat 207134.bibtex.
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- aggregation isPartOf urn:issn:0093-691X.
- aggregation language "eng".
- aggregation subject "Veterinary Sciences".
- aggregation title "Compaction rate of in vitro fertilized bovine embryos related to the interval to first cleavage".
- aggregation abstract "A study was conducted on early cleavage divisions and timing of compaction in bovine preimplantation-stage embryos. Zygotes were produced using conventional in vitro maturation and fertilization procedures. Twenty hours post insemination, the zygotes were denuded and cultured with oviduct epithelial cells in B2 medium + 10% estrous cow serum. Starting at 24 hours post insemination, the embryos (n=657) were evaluated every 6 hours and then were put into different co-culture drops according to their cell number. Starting from 78 hours post insemination, the cleavage rate was evaluated every 12 hours. Embryos were stained with Hoechst 33342 at the compacted morula stage or when they were degenerated, at 162 hours post insemination. Developmentally capable embryos were characterized by a rapid cleavage rate in the first 3 cell cycles and by an extended 8- to 16-cell stage. Peak concentrations of 2-, 4-, 8- and 16-cell stages emerged at 36, 42, 60 and 102 hours post insemination, respectively. Compaction did not occur until 126 hours post insemination. The rate of compaction was significantly higher in embryos that were at the 2-cell stage before or at 36 hours post insemination (P < 0.05). The mean cell numbers of compacted morulae that were identified at 126 and 138 hours post insemination were 30.9 +/- 6.8 and 31.6 +/-7.7, respectively. These results indicate that developmentally capable bovine embryos reach the 2-cell stage at 36 hours post insemination, and that they become compacted at the 32-cell stage, which usually occurs between 126 and 138 hours post insemination.".
- aggregation authorList BK377725.
- aggregation endPage "919".
- aggregation issue "5".
- aggregation startPage "905".
- aggregation volume "38".
- aggregation isDescribedBy 207134.
- aggregation similarTo 0093-691X(92)90165-N.
- aggregation similarTo LU-207134.