Matches in UGent Biblio for { <https://biblio.ugent.be/publication/208762#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B66289.
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- aggregation date "2002".
- aggregation format "application/pdf".
- aggregation hasFormat 208762.bibtex.
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- aggregation isPartOf urn:issn:1535-9476.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Chromatographic isolation of methionine-containing peptides for gel-free proteome analysis: identification of more than 800 Escherichia coli proteins".
- aggregation abstract "A novel gel-free proteomic technology was used to identify more than 800 proteins from 50 million Escherichia coli K12 cells in a single analysis. A peptide mixture is first obtained from a total unfractionated cell lysate, and only the methionine-containing peptides are isolated and identified by mass spectrometry and database searching. The sorting procedure is based on the concept of diagonal chromatography but adapted for highly complex mixtures. Statistical analysis predicts that we have identified more than 40% of the expressed proteome, including soluble and membrane-bound proteins. Next to highly abundant proteins, we also detected low copy number components such as the E. coli lactose operon repressor, illustrating the high dynamic range. The method is about 100 times more sensitive than two-dimensional gel-based methods and is fully automated. The strongest point, however, is the flexibility in the peptide sorting chemistry, which may target the technique toward quantitative proteomics of virtually every class of peptides containing modifiable amino acids, such as phosphopeptides, amino-terminal peptides, etc., adding a new dimension to future proteome research.".
- aggregation authorList BK167258.
- aggregation endPage "903".
- aggregation issue "11".
- aggregation startPage "896".
- aggregation volume "1".
- aggregation aggregates 3113967.
- aggregation isDescribedBy 208762.
- aggregation similarTo mcp.M200061-MCP200.
- aggregation similarTo LU-208762.