Matches in UGent Biblio for { <https://biblio.ugent.be/publication/2102757#aggregation> ?p ?o. }
Showing items 1 to 39 of
39
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B223531.
- aggregation creator B223532.
- aggregation creator B223533.
- aggregation creator B223534.
- aggregation creator B223535.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 2102757.bibtex.
- aggregation hasFormat 2102757.csv.
- aggregation hasFormat 2102757.dc.
- aggregation hasFormat 2102757.didl.
- aggregation hasFormat 2102757.doc.
- aggregation hasFormat 2102757.json.
- aggregation hasFormat 2102757.mets.
- aggregation hasFormat 2102757.mods.
- aggregation hasFormat 2102757.rdf.
- aggregation hasFormat 2102757.ris.
- aggregation hasFormat 2102757.txt.
- aggregation hasFormat 2102757.xls.
- aggregation hasFormat 2102757.yaml.
- aggregation isPartOf urn:issn:1936-9751.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Agriculture and Food Sciences".
- aggregation title "Detection of Clostridium botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay".
- aggregation abstract "Using commercially available antibodies and toxoids as templates, an ELISA, immuno-quantitative PCR (iqPCR), and multiplex immuno-PCR (iPCR) were developed for detection of Clostridium botulinum neurotoxins A and B. The obtained sensitivities for ELISA ranged from 1 ng/ml in PBS + 1% BSA to 15 and 10 ng/ml in skimmed milk for botulinum neurotoxins (BoNT)/A and BoNT/B, respectively. In semi-fat milk, the limit of detection (LOD) for both toxoids was 30 ng/ml. Quantitative immuno-PCR (iqPCR) had an LOD of 4.5-9 pg/reaction for BoNT/A in both PBS and semi-fat milk, while this was 18.5-37 pg/reaction for BoNT/B in PBS + 1% BSA and semi-fat milk, respectively. The sensitivities of ELISA and iqPCR were improved to 0.5 ng/ml and 3.75 pg/ml (0.2 pg/reaction) in semi-fat milk, respectively, when toxoid of BoNT/A was substituted with actual toxin. Multiplex iPCR with both toxoids run in the same reaction was able to distinguish presence/absence of tested BoNT/A and BoNT/B at 25 pg/reaction. The tested system offers a realistic alternative with much better sensitivity to the standard mouse assay.".
- aggregation authorList BK489557.
- aggregation endPage "326".
- aggregation issue "3".
- aggregation startPage "319".
- aggregation volume "5".
- aggregation aggregates 2103261.
- aggregation isDescribedBy 2102757.
- aggregation similarTo s12161-011-9300-7.
- aggregation similarTo LU-2102757.