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- aggregation classification "A1".
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 2111280.bibtex.
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- aggregation hasFormat 2111280.dc.
- aggregation hasFormat 2111280.didl.
- aggregation hasFormat 2111280.doc.
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- aggregation hasFormat 2111280.yaml.
- aggregation isPartOf urn:issn:0920-8569.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Instability in vitro of a PCV2 infectious clone containing an insertion between ORF1 and ORF2".
- aggregation abstract "Porcine circovirus 2 (PCV2) is a small circular single-stranded DNA virus that causes postweaning multisystemic wasting syndrome in pigs. We obtained an infectious clone, derived from PCV2 strain 1147 (size 1,767 bp), with a 60 bp insertion in the cap-rep intergenic region at the 3' end of the rep. In vitro propagation and sequencing of virus showed mixed sequence for the cap-rep intergenic region. Cloning of this region of virus from the passages 2 and 20 showed deletions of various size of the 60 bp insertion. Viruses from the passage 20 showed deletions of the 60 bp insertion and had extra (+1 to +5) or less (-1 or -2) bp in the intergenic region as compared to 1,147. These findings suggest that insertion of a 60-bp DNA sequence at the 3' end of the rep is unstable in vitro. This finding can have implications for the genetic engineering of PCV2.".
- aggregation authorList BK962058.
- aggregation endPage "261".
- aggregation issue "2".
- aggregation startPage "258".
- aggregation volume "44".
- aggregation aggregates 2111287.
- aggregation isDescribedBy 2111280.
- aggregation similarTo s11262-011-0693-6.
- aggregation similarTo LU-2111280.