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- aggregation classification "A1".
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 2964182.bibtex.
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- aggregation isPartOf urn:issn:0721-7714.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata".
- aggregation abstract "A method has been developed to genetically transform the medicinal plant Maesa lanceolata. Initially we tested conditions for transient expression of GFP bearing constructs in agroinfiltrated leaves. Leaf tissues of M. lanceolata were infiltrated with Agrobacterium tumefaciens carrying a nuclear-targeted GFP-construct to allow the quantification of the transformation efficiency. The number of transfected cells depended on the bacterial density, bacterial strains, the co-cultivation time, and presence of acetosyringone. The transient transformation assay generated the highest ratio of transfected cells over non-transfected cells upon 5 days post infiltration using A. tumefaciens strain LBA4404 at an OD600 = 1.0 in the presence of 100 µM acetosyringone and in the absence of a viral suppressor construct. In a second series of experiments we set up a stable transformation protocol that resulted in the regeneration of kanamycin resistant plants expressing nuclear GFP. This transformation protocol will be used to introduce overexpression and RNAi constructs into M. lanceolata plants that may interfere with triterpenoid saponin biosynthesis.".
- aggregation authorList BK675155.
- aggregation endPage "1526".
- aggregation issue "8".
- aggregation startPage "1517".
- aggregation volume "31".
- aggregation aggregates 2964183.
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- aggregation similarTo s00299-012-1266-4.
- aggregation similarTo LU-2964182.