Matches in UGent Biblio for { <https://biblio.ugent.be/publication/300285#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B126959.
- aggregation creator B126960.
- aggregation creator B126961.
- aggregation creator B126962.
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- aggregation creator person.
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- aggregation creator person.
- aggregation date "2005".
- aggregation format "application/pdf".
- aggregation hasFormat 300285.bibtex.
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- aggregation isPartOf urn:issn:1059-1524.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Phosphorylated BRCA1 is predominantly located in the nucleus and mitochondria".
- aggregation abstract "Multiple copies of the mitochondrial genome in eukaryotic cells are organized into protein-DNA complexes called nucleoids. Mitochondrial genome repair mechanisms have been reported, but they are less well characterized than their nuclear counterparts. To expand our knowledge of mitochondrial genome maintenance, we have studied the localization of the BRCA1 protein, known to be involved in nuclear repair pathways. Our confocal and immunoelectron microscopy results show that BRCA1 is present in mitochondria of several human cancer cell lines and in primary breast and nasal epithelial cells. BRCA1 localization in mitochondria frequently overlapped that of nucleoids. Small interfering RNA-mediated knockdown of BRCA1 in human cancer cells (confirmed by Western blot) results in decreased nuclear cytoplasmic, and mitochondrial staining after immunofluorescence microscopy, establishing the specificity of the BRCA1 immunolabeling. Furthermore, using cell fractionation, dephosphorylation, and enzyme protection experiments, we show, that a 220-kDa phosphorylated isoform of BRCA1 is enriched in mitochondrial and nuclear fractions but reduced in cytoplasmic subcellular fractions. Submitochondrial fractionation confirmed the presence of BRCA1 protein in isolated mitoplasts. Because phosphorylation of BRCA1 and subsequent changes in subcellular localization are known to follow DNA damage, our data support a universal role for BRCA1 in the maintenance of genome integrity in both mitochondria and nucleus.".
- aggregation authorList BK326117.
- aggregation endPage "1010".
- aggregation issue "2".
- aggregation startPage "997".
- aggregation volume "16".
- aggregation aggregates 1019087.
- aggregation isDescribedBy 300285.
- aggregation similarTo LU-300285.