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- aggregation classification "P1".
- aggregation creator B87739.
- aggregation creator B87740.
- aggregation creator B87741.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 3076393.bibtex.
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- aggregation hasFormat 3076393.doc.
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- aggregation isPartOf urn:isbn:9789066055957.
- aggregation isPartOf urn:issn:0567-7572.
- aggregation language "eng".
- aggregation publisher "International Society for Horticultural Science (ISHS)".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Genetic transformation of Fragaria with candidate genes involved in drought stress".
- aggregation abstract "Agrobacterium-mediated transformation of Fragaria was performed in F. vesca, F. chiloensis and F. × ananassa ‘Ventana’ and ‘EL02.2011’ to allow a functional analysis of CAT (catalase), P5CS (Δ1-pyrroline-5-carboxylate synthetase) and FasAIV (sucrose acid invertase) genes under drought stress. These genes were targeted independently for the silencing by RNAi and over-expression in Fragaria. In vitro-grown plantlets of F. vesca, F. chiloensis and F. × ananassa ‘EL02.2011’ and in vivo-grown plants of F. × ananassa ‘Ventana’ were transformed with three different RNAi constructs coding for a hairpin RNA containing inverted repeat of F. vesca sequences for these three genes. They were also transformed with one construct coding for over-expression of FaAIV whole coding sequence from F. × ananassa. Transformation for all constructs was confirmed by visible GFP expression from the same T-DNA. There was no callusing and regeneration of F. chiloensis. Callus formation was obtained in F. × ananassa ‘Ventana’ but shoot proliferation was weak. F. vesca presented reasonable callusing, shoot regeneration and transformation using kanamycin (25 mg/L) selection. F. × ananassa ‘EL02.2011’ showed high regeneration and transformation efficiency with stable GFP expression in shoots. The occurrence of transgenic plants was confirmed by PCR analysis of genomic DNA in F. vesca and F. × ananassa ‘EL02.2011’. Consistent with previous observations, here direct comparison of wild species and cultivars shows that the transformation efficiency in Fragaria can be affected by the type genotype, gene, construct and explant as well as the concentration of A. tumefaciens suspension culture.".
- aggregation authorList BK226325.
- aggregation endPage "404".
- aggregation startPage "397".
- aggregation volume "961".
- aggregation aggregates 3076409.
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