Matches in UGent Biblio for { <https://biblio.ugent.be/publication/3097643#aggregation> ?p ?o. }
Showing items 1 to 45 of
45
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B453524.
- aggregation creator B453525.
- aggregation creator B453526.
- aggregation creator B453527.
- aggregation creator B453528.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 3097643.bibtex.
- aggregation hasFormat 3097643.csv.
- aggregation hasFormat 3097643.dc.
- aggregation hasFormat 3097643.didl.
- aggregation hasFormat 3097643.doc.
- aggregation hasFormat 3097643.json.
- aggregation hasFormat 3097643.mets.
- aggregation hasFormat 3097643.mods.
- aggregation hasFormat 3097643.rdf.
- aggregation hasFormat 3097643.ris.
- aggregation hasFormat 3097643.txt.
- aggregation hasFormat 3097643.xls.
- aggregation hasFormat 3097643.yaml.
- aggregation isPartOf urn:issn:1465-6906.
- aggregation language "eng".
- aggregation rights "I have retained and own the full copyright for this publication".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Genome-wide promoter methylation analysis in neuroblastoma identifies prognostic methylation biomarkers.".
- aggregation abstract "BACKGROUND: Accurate outcome prediction in neuroblastoma, which is necessary to enable the optimal choice of risk-related therapy, remains a challenge. To improve neuroblastoma patient stratification, this study aimed to identify prognostic tumor DNA methylation biomarkers. RESULTS: To identify genes silenced by promoter methylation, we first applied two independent genome-wide methylation screening methodologies to eight neuroblastoma cell lines. Specifically, we used re-expression profiling upon 5-aza-2'-deoxycytidine (DAC) treatment and massively parallel sequencing after capturing with a methyl-CpG-binding domain (MBD-seq). Putative methylation markers were selected from DAC-upregulated genes through a literature search and an upfront methylation-specific PCR on 20 primary neuroblastoma tumors, as well as through MBD- seq in combination with publicly available neuroblastoma tumor gene expression data. This yielded 43 candidate biomarkers that were subsequently tested by high-throughput methylation-specific PCR on an independent cohort of 89 primary neuroblastoma tumors that had been selected for risk classification and survival. Based on this analysis, methylation of KRT19, FAS, PRPH, CNR1, QPCT, HIST1H3C, ACSS3 and GRB10 was found to be associated with at least one of the classical risk factors, namely age, stage or MYCN status. Importantly, HIST1H3C and GNAS methylation was associated with overall and/or event-free survival. CONCLUSIONS: This study combines two genome-wide methylation discovery methodologies and is the most extensive validation study in neuroblastoma performed thus far. We identified several novel prognostic DNA methylation markers and provide a basis for the development of a DNA methylation-based prognostic classifier in neuroblastoma.".
- aggregation authorList BK789263.
- aggregation issue "10".
- aggregation volume "13".
- aggregation aggregates 3097715.
- aggregation isDescribedBy 3097643.
- aggregation similarTo gb-2012-13-10-r95.
- aggregation similarTo LU-3097643.