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- aggregation classification "A1".
- aggregation creator B510599.
- aggregation creator B510600.
- aggregation creator B510601.
- aggregation creator B510602.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 3099107.bibtex.
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- aggregation hasFormat 3099107.doc.
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- aggregation isPartOf urn:issn:1755-098X.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "SNP discovery using paired-end RAD-tag sequencing on pooled genomic DNA of Sisymbrium austriacum (Brassicaceae)".
- aggregation abstract "Single nucleotide polymorphisms SNPs are rapidly replacing anonymous markers in population genomic studies, but their use in non model organisms is hampered by the scarcity of cost-effective approaches to uncover genome-wide variation in a comprehensive subset of individuals. The screening of one or only a few individuals induces ascertainment bias. To discover SNPs for a population genomic study of the Pyrenean rocket (Sisymbrium austriacum subsp. chrysanthum), we undertook a pooled RAD-PE (Restriction site Associated DNA Paired-End sequencing) approach. RAD tags were generated from the PstI-digested pooled genomic DNA of 12 individuals sampled across the species distribution range and paired-end sequenced using Illumina technology to produce similar to 24.5Mb of sequences, covering similar to 7% of the specie's genome. Sequences were assembled into similar to 76000 contigs with a mean length of 323bp (N50=357bp, sequencing depth=24x). In all, >15000 SNPs were called, of which 47% were annotated in putative genic regions based on homology with the Arabidopsis thaliana genome. Gene ontology (GO) slim categorization demonstrated that the identified SNPs covered extant genic variation well. The validation of 300 SNPs on a larger set of individuals using a KASPar assay underpinned the utility of pooled RAD-PE as an inexpensive genome-wide SNP discovery technique (success rate: 87%). In addition to SNPs, we discovered >600 putative SSR markers.".
- aggregation authorList BK856020.
- aggregation endPage "275".
- aggregation issue "2".
- aggregation startPage "269".
- aggregation volume "13".
- aggregation aggregates 5660299.
- aggregation isDescribedBy 3099107.
- aggregation similarTo 1755-0998.12039.
- aggregation similarTo LU-3099107.