Matches in UGent Biblio for { <https://biblio.ugent.be/publication/3130651#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B434845.
- aggregation creator person.
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- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 3130651.bibtex.
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- aggregation isPartOf urn:issn:1932-6203.
- aggregation language "eng".
- aggregation rights "I have retained and own the full copyright for this publication".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors".
- aggregation abstract "Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dynamic range of detection. In contrast, measurement of reverse transcriptase (RT) activity is a generic method which can be adapted for higher sensitivity using real-time PCR quantification (qPCR-based product-enhanced RT (PERT) assay). We present an evaluation of a modified SYBR Green I-based PERT assay (SG-PERT), using commercially available reagents such as MS2 RNA and ready-to-use qPCR mixes. This assay has a dynamic range of 7 logs, a sensitivity of 10 nU HIV-1 RT and outperforms p24 ELISA for HIV titer determination by lower inter-run variation, lower cost and higher linear range. The SG-PERT values correlate with transducing and infectious units in HIV-based viral vector and replication-competent HIV-1 preparations respectively. This assay can furthermore quantify Moloney Murine Leukemia Virus-derived vectors and can be performed on different instruments, such as Roche Lightcycler® 480 and Applied Biosystems ABI 7300. We consider this test to be an accurate, fast and relatively cheap method for retroviral quantification that is easily implemented for use in routine and research laboratories.".
- aggregation authorList BK757630.
- aggregation issue "12".
- aggregation volume "7".
- aggregation aggregates 3181041.
- aggregation isDescribedBy 3130651.
- aggregation similarTo journal.pone.0050859.
- aggregation similarTo LU-3130651.