Matches in UGent Biblio for { <https://biblio.ugent.be/publication/3223041#aggregation> ?p ?o. }
Showing items 1 to 38 of
38
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B444941.
- aggregation creator B444942.
- aggregation creator B444943.
- aggregation creator B444944.
- aggregation creator B444945.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 3223041.bibtex.
- aggregation hasFormat 3223041.csv.
- aggregation hasFormat 3223041.dc.
- aggregation hasFormat 3223041.didl.
- aggregation hasFormat 3223041.doc.
- aggregation hasFormat 3223041.json.
- aggregation hasFormat 3223041.mets.
- aggregation hasFormat 3223041.mods.
- aggregation hasFormat 3223041.rdf.
- aggregation hasFormat 3223041.ris.
- aggregation hasFormat 3223041.txt.
- aggregation hasFormat 3223041.xls.
- aggregation hasFormat 3223041.yaml.
- aggregation isPartOf urn:issn:0003-2700.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Chemistry".
- aggregation title "Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry with single internal standard and response factor approach".
- aggregation abstract "The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospray ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the nontargeted quantitation method described here, concentrations of lipid classes are obtained by the peak integration in HILIC chromatograms multiplied by their RFs related to the single internal standard (i.e., sphingosyl PE, d17:1/12:0) used as common reference for all polar lipid classes. The accuracy, reproducibility and robustness of the method have been checked by various means: (1) the comparison with conventional lipidomic quantitation using SRM scans on a triple quadrupole (QqQ) mass analyzer, (2) P-31 nuclear magnetic resonance (NMR) quantitation of the total lipid extract, (3) method robustness test using subsequent measurements by three different persons, (4) method transfer to different HPLC/MS systems using different chromatographic conditions, and (5) comparison with previously published results for identical samples, especially human reference plasma from the National Institute of Standards and Technology (NIST human plasma). Results on human plasma, egg yolk and porcine liver extracts are presented and discussed.".
- aggregation authorList BK777007.
- aggregation endPage "10070".
- aggregation issue "22".
- aggregation startPage "10064".
- aggregation volume "84".
- aggregation aggregates 3223052.
- aggregation isDescribedBy 3223041.
- aggregation similarTo ac3024476.
- aggregation similarTo LU-3223041.