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- aggregation classification "A1".
- aggregation creator B173312.
- aggregation creator B173313.
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- aggregation date "2005".
- aggregation format "application/pdf".
- aggregation hasFormat 324515.bibtex.
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- aggregation isPartOf urn:issn:0095-1137.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Evaluation of tRNA gene PCR for identification of Mollicutes".
- aggregation abstract "We evaluated the applicability of IRNA gene PCR in combination with fluorescent capillary electrophoresis with an ABI310 genetic analyzer (Applied Biosystems, Calif.) for the identification of different mollicute species. A total of 103 strains and DNA extracts of 30 different species belonging to the genera Acholeplasma, Mycoplasma, and Ureaplasma were studied. Reproducible peak profiles were generated for all samples, except for one M. genitalium isolate, the three M. gallisepticum isolates, and 8 of the 24 Ureaplasma cultures, where no amplification could be obtained. Clustering revealed numerous discrepancies compared to the identifications that had been previously obtained by means of biochemical and serological tests. Final identification was obtained by 16S rRNA gene amplification followed by sequence analysis and/or restriction digestion. This confirmed the identification obtained by tRNA gene PCR in all cases. Seven samples yielded an unexpected tRNA gene PCR profile. Sequence analysis of the 16S rRNA genes showed that six of these samples were mixed and that one had a unique sequence that did not match any of the published sequences, pointing to the existence of a not-yet-described species. In conclusion, we found IRNA gene PCR to be a rapid and discriminatory method to correctly identify a large collection of different species of the class of Mollicutes and to recognize not-yet-described groups.".
- aggregation authorList BK425585.
- aggregation endPage "4566".
- aggregation issue "9".
- aggregation startPage "4558".
- aggregation volume "43".
- aggregation aggregates 560616.
- aggregation isDescribedBy 324515.
- aggregation similarTo JCM.43.9.4558-4566.2005.
- aggregation similarTo LU-324515.