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- aggregation classification "A1".
- aggregation creator B163248.
- aggregation creator B163249.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2003".
- aggregation format "application/pdf".
- aggregation hasFormat 348594.bibtex.
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- aggregation isPartOf urn:issn:0022-0302.
- aggregation language "eng".
- aggregation publisher "AMER DAIRY SCIENCE ASSOC".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Differential leukocyte count method for bovine low somatic cell count milk".
- aggregation abstract "Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk. We have developed a flow cytometric differential leukocyte count method for low SCC milk. The procedure consists of 1) 1.5 ml of diluted milk sample (30%, vol/vol dilution with PBS), 2) centrifugation, 3) leukocyte labeling with SYTO 13 and 4) flow cytometric analysis. Four major leukocyte populations can be clearly identified in the green fluorescence-side scatter dot plot: lymphocytes and monocytes (LM), polymorphonuclear neutrophils (PMN), mature macrophages (MO), and cells with apoptotic features based on chromatin condensation and nuclear fragmentation. The optimal processing temperature was 20degreesC. Significant differences among samples with similar differential leukocyte counts were found. Storage of milk samples during 2 d at 7degreesC had no effect on differential leukocyte count. Using the new method, differential leukocyte count was performed in low SCC milk samples from cows in early, mid, and late lactation. In accordance with previous studies, PMN and M P percentages were lower and LM percentages were higher in early lactation than in the other stages of lactation. The percentage of cells with apoptotic features was higher in early lactation than in mid and late lactation. In conclusion, a rapid, simple, accurate, and reproducible standard procedure was developed to determine the differential leukocyte count (MO, PMN, LM, and cells with apoptotic features) of bovine low SCC milk.".
- aggregation authorList BK404299.
- aggregation endPage "834".
- aggregation issue "3".
- aggregation startPage "828".
- aggregation volume "86".
- aggregation aggregates 984897.
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