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- aggregation classification "A1".
- aggregation creator B379209.
- aggregation creator B379210.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2007".
- aggregation format "application/pdf".
- aggregation hasFormat 382558.bibtex.
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- aggregation isPartOf urn:issn:0928-4249.
- aggregation language "eng".
- aggregation publisher "EDP SCIENCES S A".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis".
- aggregation abstract "The dynamics of blood neutrophil acyloxyacyl hydrolase ( AOAH) activity, the appearance of endotoxin ( lipopolysaccharide, LPS) in blood and the role of blood neutrophil AOAH in the severity of Escherichia coli and endotoxin mastitis were investigated in early postpartum dairy cows experimentally challenged with either endotoxin ( n = 6) or E. coli ( n = 6). The AOAH activity of blood neutrophils started to decrease significantly at post challenge hours ( PCH) 6 - 24 and 12 - 24 in the endotoxin and E. coli-challenged groups, respectively; it returned to pre-challenged values at PCH 48 in both endotoxin- and E. coli-challenged groups. The cows were classified as moderate and severe responders according to milk production loss in the non-challenged quarters at PCH 48. There were no severe responders in the endotoxin- challenged group. In the E. coli-challenged group, only 1 severe responder was identified. The pre-challenge neutrophil AOAH activity of the severe responder was similar to 30% lower than that of moderate responders. No LPS was detected in the plasma of endotoxin- challenged cows; neither was it found in the plasma of moderate responders in the E. coli-challenged group at any PCH. However, at PCH 6, a remarkable amount of LPS was detected in the plasma of the severe responder from the E. coli-challenged group. Furthermore, neutrophil AOAH activity was increased by similar to 70% in the severe responder at PCH 6, but it increased by only similar to 15% in moderate responders. This was followed by a decreased neutrophil AOAH activity at PCH 12 - 24 and 24 - 72 in moderate and severe responders, respectively; the decreased AOAH activity at those PCH was more pronounced in the severe responder. The pronounced decreased neutrophil AOAH activity during mastitis often coincided with extreme leukopenia, neutropenia and a maximal number of immature neutrophils in the blood. Our results demonstrate that a decrease in neutrophil AOAH activity results in the appearance of LPS in the blood, and low blood neutrophil deacylation activity could be considered as a risk factor for severe clinical coliform mastitis.".
- aggregation authorList BK685203.
- aggregation endPage "668".
- aggregation issue "5".
- aggregation startPage "655".
- aggregation volume "38".
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