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- aggregation classification "A1".
- aggregation creator person.
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- aggregation date "2007".
- aggregation format "application/pdf".
- aggregation hasFormat 384638.bibtex.
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- aggregation isPartOf urn:issn:1618-2642.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Raman spectroscopic study of bacterial endospores".
- aggregation abstract "Endospores and endospore-forming bacteria were studied by Raman spectroscopy. Raman spectra were recorded from Bacillus licheniformis LMG 7634 at different steps during growth and spore formation, and from spore suspensions obtained from diverse Bacillus and Paenibacillus strains cultured in different conditions (growth media, temperature, peroxide treatment). Raman bands of calcium dipicolinate and amino acids such as phenylalanine and tyrosine are more intense in the spectra of sporulating bacteria compared with those of bacteria from earlier phases of growth. Raman spectroscopy can thus be used to detect sporulation of cells by a characteristic band at 1,018 cm(-1) from calcium dipicolinate. The increase in amino acids could possibly be explained by the formation of small acid-soluble proteins that saturate the endospore DNA. Large variations in Raman spectra of endospore suspensions of different strains or different culturing conditions were observed. Next to calcium dipicolinate, tyrosine and phenylalanine, band differences at 527 and 638 cm(-1) were observed in the spectra of some of the B. sporothermodurans spore suspensions. These bands were assigned to the incorporation of cysteine residues in spore coat proteins. In conclusion, Raman spectroscopy is a fast technique to provide useful information about several spore components.".
- aggregation authorList BK576044.
- aggregation endPage "2151".
- aggregation issue "7-8".
- aggregation startPage "2143".
- aggregation volume "389".
- aggregation aggregates 1929928.
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- aggregation similarTo s00216-007-1616-1.
- aggregation similarTo LU-384638.