Matches in UGent Biblio for { <https://biblio.ugent.be/publication/387813#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B443144.
- aggregation creator person.
- aggregation creator person.
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- aggregation date "2007".
- aggregation format "application/pdf".
- aggregation hasFormat 387813.bibtex.
- aggregation hasFormat 387813.csv.
- aggregation hasFormat 387813.dc.
- aggregation hasFormat 387813.didl.
- aggregation hasFormat 387813.doc.
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- aggregation hasFormat 387813.txt.
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- aggregation isPartOf urn:issn:8756-7938.
- aggregation language "eng".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Comparison of different strategies to reduce acetate formation in Escherichia coli".
- aggregation abstract "E. coli cells produce acetate as an extracellular coproduct of aerobic cultures. Acetate is undesirable because it retards growth and inhibits protein formation. Most process designs or genetic modifications to minimize acetate formation aim at balancing growth rate and oxygen consumption. In this research, three genetic approaches to reduce acetate formation were investigated: (1) direct reduction of the carbon flow to acetate (ackA-pta, poxB knock-out); (2) anticipation on the underlying metabolic and regulatory mechanisms that lead to acetate (constitutive ppc expression mutant); and (3) both (1) and (2). Initially, these mutants were compared to the wild-type E. coli via batch cultures under aerobic conditions. Subsequently, these mutants were further characterized using metabolic flux analysis on continuous cultures. It is concluded that a combination of directly reducing the carbon flow to acetate and anticipating on the underlying metabolic and regulatory mechanism that lead to acetate, is the most promising approach to overcome acetate formation and improve recombinant protein production. These genetic modifications have no significant influence on the metabolism when growing the microorganisms under steady state at relatively low dilution rates (less than 0.4 h(-1)).".
- aggregation authorList BK773570.
- aggregation endPage "1063".
- aggregation issue "5".
- aggregation startPage "1053".
- aggregation volume "23".
- aggregation aggregates 876267.
- aggregation isDescribedBy 387813.
- aggregation similarTo bp070170g.
- aggregation similarTo LU-387813.