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- aggregation classification "P1".
- aggregation creator person.
- aggregation creator person.
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- aggregation creator person.
- aggregation creator person.
- aggregation date "1998".
- aggregation hasFormat 399761.bibtex.
- aggregation hasFormat 399761.csv.
- aggregation hasFormat 399761.dc.
- aggregation hasFormat 399761.didl.
- aggregation hasFormat 399761.doc.
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- aggregation hasFormat 399761.mods.
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- aggregation hasFormat 399761.txt.
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- aggregation hasFormat 399761.yaml.
- aggregation isPartOf urn:issn:1373-7503.
- aggregation language "eng".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants".
- aggregation abstract "Plant-parasitic nematodes are among the most important pests of agricultural plants worldwide. They infect plants and establish complex feeding sites inside the root tissues. Plant-parasitic nematodes are controlled to same extent using conventional methods, These methods are either not efficient or cause environmental hazard. An alternative and promising way is making nematode-resistant plants by introducing engineered genes. Using die promoter tagging approach, we have identified some nematode-inducible plant promoters. After isolation of these promoters, they have been fused to the beta-glucuronidase (gus) marker. We have introduced the promoter-gus fusions into different plant species via Agrobacterium tumefaciens or Agrobacterium rhizogenes. Since plant-parasitic nematodes invade the plant root, we found re-introduction of plant promoters by using A. rhizogenes relatively faster and easier. To get a hgh frequency of transformation and hairy root regeneration, plant media and hormone compositions mere optimised. Then a number of plant species were transformed using A. rhizogenes pRil5834. The Agrobacterium harboured a binary vector containing a T-DNA composed of a nematode-inducible promoter gus-fusion and nptII gene. Integration of the T-DNAs from the binary vector and the Ri plasmid results in the generation of kanamycin-resistant hairy roots.".
- aggregation authorList BK157706.
- aggregation endPage "617".
- aggregation issue "3a".
- aggregation startPage "609".
- aggregation volume "63".
- aggregation isDescribedBy 399761.
- aggregation similarTo LU-399761.