Matches in UGent Biblio for { <https://biblio.ugent.be/publication/4090605#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B866776.
- aggregation creator B866777.
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- aggregation creator B866779.
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- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 4090605.bibtex.
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- aggregation isPartOf urn:issn:0340-3696.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Identifying targets for topical RNAi therapeutics in psoriasis: assessment of a new in vitro psoriasis model".
- aggregation abstract "Diseases of the skin are amenable to RNAi-based therapies and targeting key components in the pathophysiology of psoriasis using RNAi may represent a successful new therapeutic strategy. We aimed to develop a straightforward and highly reproducible in vitro psoriasis model useful to study the effects of gene knockdown by RNAi and to identify new targets for topical RNAi therapeutics. We evaluated the use of keratinocytes derived from psoriatic plaques and normal human keratinocytes (NHKs). To induce a psoriatic phenotype in NHKs, combinations of pro-inflammatory cytokines (IL-1 alpha, IL-17A, IL-6 and TNF-alpha) were tested. The model based on NHK met our needs of a reliable and predictive preclinical model, and this model was further selected for gene expression analyses, comprising a panel of 55 psoriasis-associated genes and five micro-RNAs (miRNAs). Gene silencing studies were conducted by using small interfering RNAs (siRNAs) and miRNA inhibitors directed against potential target genes such as CAMP and DEFB4 and miRNAs such as miR-203. We describe a robust and highly reproducible in vitro psoriasis model that recapitulates expression of a large panel of genes and miRNAs relevant to the pathogenesis of psoriasis. Furthermore, we show that our model is a powerful first step model system for testing and screening RNAi-based therapeutics.".
- aggregation authorList BK1244382.
- aggregation endPage "512".
- aggregation issue "6".
- aggregation startPage "501".
- aggregation volume "305".
- aggregation aggregates 5874499.
- aggregation isDescribedBy 4090605.
- aggregation similarTo s00403-013-1379-9.
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