Matches in UGent Biblio for { <https://biblio.ugent.be/publication/4158065#aggregation> ?p ?o. }
Showing items 1 to 42 of
42
with 100 items per page.
- aggregation classification "A1".
- aggregation creator B165989.
- aggregation creator B165990.
- aggregation creator B165991.
- aggregation creator B165992.
- aggregation creator B165993.
- aggregation creator B165994.
- aggregation creator B165995.
- aggregation creator B165996.
- aggregation creator B165997.
- aggregation creator B165998.
- aggregation creator B165999.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 4158065.bibtex.
- aggregation hasFormat 4158065.csv.
- aggregation hasFormat 4158065.dc.
- aggregation hasFormat 4158065.didl.
- aggregation hasFormat 4158065.doc.
- aggregation hasFormat 4158065.json.
- aggregation hasFormat 4158065.mets.
- aggregation hasFormat 4158065.mods.
- aggregation hasFormat 4158065.rdf.
- aggregation hasFormat 4158065.ris.
- aggregation hasFormat 4158065.txt.
- aggregation hasFormat 4158065.xls.
- aggregation hasFormat 4158065.yaml.
- aggregation isPartOf urn:issn:1932-6203.
- aggregation language "eng".
- aggregation rights "I have retained and own the full copyright for this publication".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Phylogenetic characterization of β-tubulins and development of pyrosequencing assays for benzimidazole resistance in cattle nematodes".
- aggregation abstract "Control of helminth infections is a major task in livestock production to prevent health constraints and economic losses. However, resistance to established anthelmintic substances already impedes effective anthelmintic treatment in many regions worldwide. Thus, there is an obvious need for sensitive and reliable methods to assess the resistance status of at least the most important nematode populations. Several single nucleotide polymorphisms (SNPs) in the beta-tubulin isotype 1 gene of various nematodes correlate with resistance to benzimidazoles (BZ), a major anthelmintic class. Here we describe the full-length beta-tubulin isotype 1 and 2 and alpha-tubulin coding sequences of the cattle nematode Ostertagia ostertagi. Additionally, the Cooperia oncophora alpha-tubulin coding sequence was identified. Phylogenetic maximum-likelihood analysis revealed that both isotype 1 and 2 are orthologs to the Caenorhabditis elegans ben-1 gene which is also associated with BZ resistance upon mutation. In contrast, a Trichuris trichiura cDNA, postulated to be beta-tubulin isotype 1 involved in BZ resistance in this human parasite, turned out to be closely related to C. elegans beta-tubulins tbb-4 and mec-7 and would therefore represent the first non-ben-1-like beta-tubulin to be under selection through treatment with BZs. A pyrosequencing assay was established to detect BZ resistance associated SNPs in beta-tubulin isotype 1 codons 167, 198 and 200 of C. oncophora and O. ostertagi. PCR-fragments representing either of the two alleles were combined in defined ratios to evaluate the pyrosequencing assay. The correlation between the given and the measured allele frequencies of the respective SNPs was very high. Subsequently laboratory isolates and field populations with known resistance status were analyzed. With the exception of codon 167 in Cooperia, increases of resistance associated alleles were detected for all codons in at least one of the phenotypically resistant population. Pyrosequencing provides a fast, inexpensive and sensitive alternative to conventional resistance detection methods.".
- aggregation authorList BK410247.
- aggregation issue "8".
- aggregation volume "8".
- aggregation aggregates 4158080.
- aggregation isDescribedBy 4158065.
- aggregation similarTo journal.pone.0070212.
- aggregation similarTo LU-4158065.