Matches in UGent Biblio for { <https://biblio.ugent.be/publication/4213018#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B176714.
- aggregation creator B176715.
- aggregation creator B176716.
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- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 4213018.bibtex.
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- aggregation isPartOf urn:issn:1550-8943.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells".
- aggregation abstract "The conditional Cre/loxP system and/or the doxycycline (Dox) inducible Tet-on/off system are widely used in mouse transgenesis but often require time consuming, inefficient cloning/screening steps and extensive mouse breeding strategies. We have therefore developed a highly efficient Gateway- and recombinase-mediated cassette exchange (RMCE)-compatible system to target conditional and/or inducible constructs to the ROSA26 locus of F1 hybrid Bl6/129 ESCs, called G4 ROSALUC ESCs. By combining the Cre/loxP system with or without the inducible Tet-on system using Gateway cloning, we can rapidly generate spatial and/or temporal controllable gain-of-function constructs that can be targeted to the RMCE-compatible ROSA26 locus of the G4 ROSALUC ESCs with efficiencies close to 100 %. These novel ESC-based technologies allow for the creation of multiple gain-of-function conditional and/or inducible transgenic ESC clones and mouse lines in a highly efficient and locus specific manner. Importantly, incorporating insulator sequences into the Dox-inducible vector system resulted in robust, stable transgene expression in undifferentiated ESCs but could not fully overcome transgene mosaicism in the differentiated state.".
- aggregation authorList BK432133.
- aggregation endPage "785".
- aggregation issue "6".
- aggregation startPage "774".
- aggregation volume "9".
- aggregation aggregates 4213046.
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