Matches in UGent Biblio for { <https://biblio.ugent.be/publication/4231230#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B838387.
- aggregation creator B838388.
- aggregation creator B838389.
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- aggregation creator B838391.
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- aggregation date "2013".
- aggregation format "application/pdf".
- aggregation hasFormat 4231230.bibtex.
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- aggregation isPartOf urn:issn:0003-2700.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Chemistry".
- aggregation title "Quantum dot loaded liposomes as fluorescent labels for immunoassay".
- aggregation abstract "Liposomes loaded with water-soluble and water-insoluble quantum dots (QD) were for the first time applied as labels in different heterogeneous immunoassays for the determination of food contaminants, using mycotoxin zearalenone (ZEN) as a model. A great deal of work was devoted to the optimal choice of phospholipids for the liposomes preparation and to the factors which are important for the stability and size of obtained liposomes. Thin-film hydration and reverse-phase evaporation techniques were evaluated in terms of stability of the obtained liposomes and their efficiency for QD loading. Conjugation of liposomes with proteins and the influence of cross-linkers to the nonspecific interaction of the obtained liposomes with the surface of microtiter plates and cartridges were investigated and 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester was found as the optimal cross-linker. The limits of detection (LOD) for ZEN of fluorescence-labeled immunosorbent assays were 0.6 mu g kg(-1), 0.08 mu g kg(-1), and 0.02 mu g kg(-1), using QD, liposomes loaded with water-soluble QD, and water-insoluble QD, respectively. Similarly, the developed qualitative on-site tests using the different QD labels and taking into account the EU maximum residues level for ZEN in unprocessed cereals showed cutoff levels of 100, 50, and 20 mu g kg(-1).".
- aggregation authorList BK1213457.
- aggregation endPage "7204".
- aggregation issue "15".
- aggregation startPage "7197".
- aggregation volume "85".
- aggregation aggregates 4231746.
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