Matches in UGent Biblio for { <https://biblio.ugent.be/publication/4251028#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B885191.
- aggregation creator B885192.
- aggregation creator B885193.
- aggregation creator B885194.
- aggregation creator B885195.
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- aggregation creator B885199.
- aggregation creator person.
- aggregation date "2012".
- aggregation format "application/pdf".
- aggregation hasFormat 4251028.bibtex.
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- aggregation isPartOf urn:issn:1937-3384.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Evaluation of the cell viability of human Wharton's jelly stem cells for use in cell therapy".
- aggregation abstract "Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity. We employed a combination of highly sensitive techniques to determine the average cell viability levels and proliferation capabilities of 10 consecutive cell passages of cultured HWJSCs and then used RNA microarrays to identify genes associated with changes in cell viability levels. We found an initial decrease in cell viability from the first to the third cell passage followed by an increase until the sixth passage and a final decrease from the sixth to tenth cell passages. The highest cell viability levels corresponded to the fifth and sixth passages. The intracellular ionic contents of potassium, sodium, and chlorine suggest that the lower cell viability levels at passages 2, 3, and 8-10 may be associated with apoptotic cell death. In fact, gene expression analysis revealed that the average cell viability was significantly associated with genes with a function in apoptotic cell death, especially pro-apoptotic FASTKD2, BNIP3L genes and anti-apoptotic TNFAIP8 and BCL2L2 genes. This correlation with both pro-apoptotic and anti-apoptotic genes suggests that there may be a complex live-death equilibrium in cultured HWJSCs kept in culture for multiple cell passages. In this study, the highest cell viability levels corresponded to the fifth and sixth HWJSC passages, suggesting that these passages should be preferentially employed in cell therapy or tissue engineering protocols using this cell type.".
- aggregation authorList BK1264603.
- aggregation endPage "419".
- aggregation issue "6".
- aggregation startPage "408".
- aggregation volume "18".
- aggregation aggregates 4251062.
- aggregation isDescribedBy 4251028.
- aggregation similarTo ten.tec.2011.0508.
- aggregation similarTo LU-4251028.