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- aggregation classification "C3".
- aggregation creator person.
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- aggregation creator person.
- aggregation date "2013".
- aggregation hasFormat 4252083.bibtex.
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- aggregation isPartOf urn:isbn:9786056393501.
- aggregation language "eng".
- aggregation publisher "Hacettepe University. Food Engineering Department".
- aggregation subject "Chemistry".
- aggregation title "The analytical performance of high resolution Orbitrap mass spectrometry for the detection and quantitation of carotenoids in tomato fruit".
- aggregation abstract "Carotenoids are important secondary metabolites, which are mainly synthesized by plants, algae and certain types of bacteria and fungi. In epidemiological and clinical studies, associations were found between the intake of the concerned dietary compounds and the prevalence of chronic-degenerative diseases, including cancers, cardiovascular disorders and age-related macular degeneration. Because of these beneficial health effects, carotenoids are intensively studied to further clarify and better understand their specific role and actions. Additionally, a further deepening of the knowledge about the metabolism and partitioning of carotenoids in plants is required, in particular when trying to increase carotenoid levels in fruits and vegetables by modifying environmental factors, cultivation techniques, or genetic manipulation. Within this context, metabolomic profiling techniques are highly relevant since they can reveal a comprehensive overview of the relative levels of hundreds to thousand metabolites, present in the plant material of interest. As such, metabolomic profiling techniques have the potential to reveal the significance of a certain metabolite with regard to a specific process and are considered to be strongly complimentary with genetic and functional genomics approaches. Therefore, during this study, a full-scan, high-resolution Fourier Transform Orbitrap MS detection method was developed for the metabolomic profiling of carotenoids in tomato fruit. The development and validation of the analytical method was based on five carotenoids, representing both carotenes and xanthophylls. In addition, the analytical performance of the novel method was comprehensively evaluated by means of a comparative study with consideration of well-established carotenoid detection techniques. For this purpose, analytical methods were optimized whereby detection was based upon either UV-vis absorption, MS/MS or MSn. By comparative evaluation of the proposed methods, information could be provided about the suitability of a certain analytical technique within a defined experimental framework and about it’s limitations and major considerations. The results with regard to the Orbitrap MS analytical method, allowing the metabolomic profiling of carotenoids in tomato fruit, and the outcome of the comparative study with other detection techniques will be presented.".
- aggregation authorList BK343879.
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