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- aggregation classification "A1".
- aggregation creator B617471.
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- aggregation date "2014".
- aggregation format "application/pdf".
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- aggregation isPartOf urn:issn:0099-2240.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Effects of growth medium on matrix-assisted laser desorption/ionization time-of-flight mass spectra: a case study of acetic acid bacteria".
- aggregation abstract "The effect of the growth medium used on the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra generated and its consequences for species and strain level differentiation of acetic acid bacteria (AAB) were determined by using a set of 25 strains. The strains were grown on five different culture media that yielded a total of more than 600 mass spectra, including technical and biological replicates. The results demonstrate that the culture medium can have a profound effect on the mass spectra of AAB as observed in the presence and varying signal intensities of peak classes, in particular when culture media do not sustain optimal growth. The observed growth medium effects do not disturb species level differentiation but strongly affect the potential for strain level differentiation. The data prove that a well-constructed and robust MALDI-TOF mass spectrometry identification database should comprise mass spectra of multiple reference strains per species grown on different culture media to facilitate species and strain level differentiation.".
- aggregation authorList BK977067.
- aggregation endPage "1538".
- aggregation issue "4".
- aggregation startPage "1528".
- aggregation volume "80".
- aggregation aggregates 4354918.
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