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- aggregation classification "A1".
- aggregation creator B843022.
- aggregation creator B843023.
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- aggregation date "2014".
- aggregation format "application/pdf".
- aggregation hasFormat 4396640.bibtex.
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- aggregation isPartOf urn:issn:2040-3364.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Physics and Astronomy".
- aggregation title "On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids".
- aggregation abstract "Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.".
- aggregation authorList BK1219780.
- aggregation endPage "1747".
- aggregation issue "3".
- aggregation startPage "1741".
- aggregation volume "6".
- aggregation aggregates 4396681.
- aggregation isDescribedBy 4396640.
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