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- aggregation classification "A1".
- aggregation creator person.
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- aggregation date "2014".
- aggregation format "application/pdf".
- aggregation hasFormat 4413115.bibtex.
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- aggregation isPartOf urn:issn:0177-5103.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Veterinary Sciences".
- aggregation title "Development of a bacterial challenge test for gnotobiotic Nile tilapia Oreochromis niloticus larvae".
- aggregation abstract "Gastrointestinal microbiota have an important impact on fish health and disease, stimulating interest in a better understanding of how these gastrointestinal microbial communities are composed and consequently affect host fitness. In this respect, probiotic microorganisms have been extensively used in recent aquaculture production. To study the use of probiotics in the treat- ment of infectious diseases, the establishment of a method of experimental infection to obtain con- sistent results for mortality and infection in challenge tests is important. In pathogen-screening tests, 4 candidate pathogenic bacteria strains (Edwardsiella ictaluri gly09, E. ictaluri gly10, E. tarda LMG2793 and Streptococcus agalactiae LMG15977) were individually tested on xenic Nile tilapia larvae. Only Edwardsiella strains delivered via Artemia nauplii, with or without additional pathogen delivery via the culture water, led to increased mortality in fish larvae. A gnotobiotic Nile tilapia larvae model system was developed to provide a research tool to investigate the effects and modes-of-action of probiotics under controlled conditions. A double disinfection pro- cedure using hydrogen peroxide and sodium hypochlorite solution was applied to the fish eggs, which were subsequently incubated in a cocktail of antibiotic and antifungal agents. In the gnoto- biotic challenge test, E. ictaluri gly09R was added to the model system via Artemia nauplii and culture water, resulting in a significant mortality of the gnotobiotic fish larvae. The developed gnotobiotic Nile tilapia model can be used as a tool to extend understanding of the mechanisms involved in host−microbe interactions and to evaluate new methods of disease control.".
- aggregation authorList BK1431556.
- aggregation endPage "34".
- aggregation issue "1".
- aggregation startPage "23".
- aggregation volume "109".
- aggregation aggregates 4413116.
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