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- aggregation classification "A1".
- aggregation creator person.
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- aggregation date "2009".
- aggregation format "application/pdf".
- aggregation hasFormat 529736.bibtex.
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- aggregation isPartOf urn:issn:0168-3659.
- aggregation language "eng".
- aggregation publisher "Elsevier".
- aggregation rights "I don't know the status of the copyright for this publication".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes".
- aggregation abstract "The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments.".
- aggregation authorList BK781372.
- aggregation endPage "220".
- aggregation issue "3".
- aggregation startPage "214".
- aggregation volume "133".
- aggregation aggregates 529964.
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- aggregation similarTo j.jconrel.2008.10.003.
- aggregation similarTo LU-529736.