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- aggregation classification "A1".
- aggregation creator B936705.
- aggregation creator B936706.
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- aggregation date "1981".
- aggregation format "application/pdf".
- aggregation hasFormat 5673051.bibtex.
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- aggregation isPartOf urn:issn:0022-2836.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Biology and Life Sciences".
- aggregation title "Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens".
- aggregation abstract "A detailed physical map of the homologous and non-homologous regions between an octopine (pTiAch5) and a nopaline (pTiC58) Ti plasmid was determined by Southern type hybridization and by electron microscope heteroduplex analysis. This map was correlated with the functional maps of both plasmids. For the Southern type hybridizations, total labelled pTiAch5 DNA was hybridized to Southern blots of restriction fragments from a series of hybrid plasmids containing overlapping segments of the whole TiC58 plasmid. Reciprocal experiments were also carried out. The common sequences between the two plasmids (±30%) are restricted to four major stretches of homology. Analysis of heteroduplexes between pTiAch5 and several hybrid plasmids containing specific regions of pTiC58, and of heteroduplexes between hybrid plasmids derived from pTiC58 and pTiAch5 provided a detailed map of the fine structure of the four major homology regions. Two regions are distributed in the same relative order as compared to a common reference point, and two are inversed. Three regions contain a number of small, mostly asymmetrical substitution loops. Several regions distributed over the common DNA sequences were found to be partially homologous.".
- aggregation authorList BK1322978.
- aggregation endPage "208".
- aggregation issue "2".
- aggregation startPage "183".
- aggregation volume "152".
- aggregation aggregates 5673122.
- aggregation isDescribedBy 5673051.
- aggregation similarTo 0022-2836(81)90239-4.
- aggregation similarTo LU-5673051.