Matches in UGent Biblio for { <https://biblio.ugent.be/publication/5775964#aggregation> ?p ?o. }
Showing items 1 to 37 of
37
with 100 items per page.
- aggregation classification "A2".
- aggregation creator B928144.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2014".
- aggregation format "application/pdf".
- aggregation hasFormat 5775964.bibtex.
- aggregation hasFormat 5775964.csv.
- aggregation hasFormat 5775964.dc.
- aggregation hasFormat 5775964.didl.
- aggregation hasFormat 5775964.doc.
- aggregation hasFormat 5775964.json.
- aggregation hasFormat 5775964.mets.
- aggregation hasFormat 5775964.mods.
- aggregation hasFormat 5775964.rdf.
- aggregation hasFormat 5775964.ris.
- aggregation hasFormat 5775964.txt.
- aggregation hasFormat 5775964.xls.
- aggregation hasFormat 5775964.yaml.
- aggregation isPartOf urn:issn:2001-3078.
- aggregation language "eng".
- aggregation rights "I have retained and own the full copyright for this publication".
- aggregation subject "Medicine and Health Sciences".
- aggregation title "The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling".
- aggregation abstract "Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer and their use as biomarkers for diagnosis, prognosis, drug response and recurrence, there is no consensus on dependable isolation protocols. We provide a comparative evaluation of 4 exosome isolation protocols for their usability, yield and purity, and their impact on downstream omics approaches for biomarker discovery. OptiPrep density gradient centrifugation outperforms ultracentrifugation and ExoQuick and Total Exosome Isolation precipitation in terms of purity, as illustrated by the highest number of CD63-positive nanovesicles, the highest enrichment in exosomal marker proteins and a lack of contaminating proteins such as extracellular Argonaute-2 complexes. The purest exosome fractions reveal a unique mRNA profile enriched for translation, ribosome, mitochondrion and nuclear lumen function. Our results demonstrate that implementation of high purification techniques is a prerequisite to obtain reliable omics data and identify exosome-specific functions and biomarkers.".
- aggregation authorList BK1311412.
- aggregation volume "3".
- aggregation aggregates 5775977.
- aggregation isDescribedBy 5775964.
- aggregation similarTo jev.v3.24858.
- aggregation similarTo LU-5775964.