Matches in UGent Biblio for { <https://biblio.ugent.be/publication/595709#aggregation> ?p ?o. }
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- aggregation classification "A1".
- aggregation creator B190250.
- aggregation creator B190251.
- aggregation creator B190252.
- aggregation creator B190253.
- aggregation creator B190254.
- aggregation creator B190255.
- aggregation creator B190256.
- aggregation creator B190257.
- aggregation creator person.
- aggregation date "2009".
- aggregation format "application/pdf".
- aggregation hasFormat 595709.bibtex.
- aggregation hasFormat 595709.csv.
- aggregation hasFormat 595709.dc.
- aggregation hasFormat 595709.didl.
- aggregation hasFormat 595709.doc.
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- aggregation hasFormat 595709.txt.
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- aggregation hasFormat 595709.yaml.
- aggregation isPartOf urn:issn:0006-291X.
- aggregation language "eng".
- aggregation title "High-throughput quantitative analysis of plant N-glycan using a DNA sequencer".
- aggregation abstract "High-throughput quantitative analytical method for plant N-glycan has been developed. All steps, including peptide N-glycosidase (PNGase) A treatment, glycan preparation, and exoglycosidase digestion, were optimized for high-throughput applications using 96-well format procedures and automatic analysis on a DNA sequencer. The glycans of horseradish peroxidase with plant-specific core alpha(1,3)-fucose can be distinguished by the comparison of the glycan profiles obtained via PNGase A and F treatments. The peaks of the glycans with (91%) and without (1.2%) alpha(1,3)-fucose could be readily quantified and shown to harbor bisecting beta(1,2)-xylose via simultaneous treatment with alpha(1,3)-mannosidase and beta(1,2)-xylosidase. This optimized method was successfully applied to analyze N-glycans of plant-expressed recombinant antibody, which was engineered to contain a minor amount of glycan harboring P(1,2)-xylose. These results indicate that our DNA sequencer-based method provides quantitative information for plant-specific N-glycan analysis in a high-throughput manner, which has not previously been achieved by glycan profiling based on mass spectrometry.".
- aggregation authorList BK448664.
- aggregation endPage "229".
- aggregation issue "2".
- aggregation startPage "223".
- aggregation volume "380".
- aggregation aggregates 2936674.
- aggregation isDescribedBy 595709.
- aggregation similarTo j.bbrc.2009.01.070.
- aggregation similarTo LU-595709.