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- aggregation classification "A1".
- aggregation creator B438856.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2008".
- aggregation format "application/pdf".
- aggregation hasFormat 606895.bibtex.
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- aggregation isPartOf urn:issn:0739-4462.
- aggregation language "eng".
- aggregation rights "I have transferred the copyright for this publication to the publisher".
- aggregation subject "Agriculture and Food Sciences".
- aggregation title "Selection for resistance to methoxyfenozide and 20-hydroxyecdysone in cells of the beet armyworm Spodoptera exigua".
- aggregation abstract "In this report with an ecdysteroid-responsive cell line of the beet armyworm, Spodoptera exigua (Se4) selection for resistance against methoxyfenozide and the insect moulting hormone (20-hydroxyecdysone, 20E) was carried out to analyze the resulting resistant cells in order to elucidate possible mechanisms of resistance towards these compounds. From these cultures, five methoxyfenozide- and four 20E-resistant subclones were selected starting from 0.1 nM methoxyfenozide up to 100 mu M and from 10 nM 20E up to 100 mu M, respectively. To date, the selected cells kept their loss of susceptibility for 100 PM. Here we evaluated two processes known to be important in insecticide resistance, namely metabolism and pharmacokinetics, in the selected methoxyfenozide- and 20E-resistant subclones. Synergism experiments with piperonyl butoxide, S,S,S-tributyl phosphorotrithioate, and diethyl maleate, which are respective inhibitors of monooxygenases, esterases, and gluthation-S-transferases, did not affect the level of the resistance. To check the possible existence of active transport in the resistant cells, we used ouabain, an inhibitor of active membrane transport. In parallel, the absorption profile was studied in resistant and susceptible cells with use of C-14-methoxyfenozide. Interestingly, resistant subclones showed cross-resistance towards methoxyfenozide and 20E. The resistance was irreversible even after the compounds were removed from the medium.".
- aggregation authorList BK765645.
- aggregation endPage "49".
- aggregation issue "1".
- aggregation startPage "36".
- aggregation volume "67".
- aggregation aggregates 606896.
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- aggregation similarTo arch.20220.
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