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- aggregation classification "A1".
- aggregation creator B330832.
- aggregation creator B330833.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2009".
- aggregation format "application/pdf".
- aggregation hasFormat 626943.bibtex.
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- aggregation isPartOf urn:issn:0266-8254.
- aggregation language "eng".
- aggregation title "An alternative real-time PCR method to detect Bacillus cereus group in naturally contaminated food gelatine: a comparison study".
- aggregation abstract "Comparison of an internally controlled real-time PCR assay with the standard plate-based assay (ISO 21871) for the detection of Bacillus cereus group cells in gelatine. A comprehensive TaqMan probe was designed allowing the real-time PCR assay to be fully inclusive and exclusive. An internal amplification control was designed and implemented at 500 copies per reaction without impact on target detection. Specific and selective detection of target cells was achieved with a quick and simple DNA preparation procedure. No significant difference (kappa = 0.99) was observed between the performance of the real-time PCR and the standard plate-based method on naturally contaminated gelatines (n = 197). Relative accuracy, relative sensitivity and relative specificity were >= 99%. The real-time PCR assay is a valid alternative of the standard plate-based assay. The real-time PCR assay decreased the time between sample collection and result from 2 days to 2 h, while analysis cost did not increase. The gelatine-producing industry can ensure gelatine safety and quality in a much faster way.".
- aggregation authorList BK623939.
- aggregation endPage "104".
- aggregation startPage "97".
- aggregation volume "48".
- aggregation aggregates 628375.
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- aggregation similarTo j.1472-765X.2008.02495.x.
- aggregation similarTo LU-626943.