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- aggregation classification "B2".
- aggregation creator B1013043.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2009".
- aggregation hasFormat 631538.bibtex.
- aggregation hasFormat 631538.csv.
- aggregation hasFormat 631538.dc.
- aggregation hasFormat 631538.didl.
- aggregation hasFormat 631538.doc.
- aggregation hasFormat 631538.json.
- aggregation hasFormat 631538.mets.
- aggregation hasFormat 631538.mods.
- aggregation hasFormat 631538.rdf.
- aggregation hasFormat 631538.ris.
- aggregation hasFormat 631538.txt.
- aggregation hasFormat 631538.xls.
- aggregation hasFormat 631538.yaml.
- aggregation isPartOf urn:isbn:978-1-58829-937-6.
- aggregation isPartOf urn:issn:1064-3745.
- aggregation language "eng".
- aggregation publisher "Humana Press - Springer".
- aggregation subject "Chemistry".
- aggregation title "C-Terminal Sequence Analysis of 2DE-Separated Proteins".
- aggregation abstract "The overall study of post-translational modifications (PTMs) of proteins is gaining strong interest. Beside phosphorylation and glycosylation, truncations of the nascent polypeptide chain at the N- or C-terminus are by far the most common types of PTMs. Nevertheless, little attention has been paid to the development of approaches that allow a systematic analysis of these proteolytic processing events. Here we present a protocol that allows the identification of the C-terminal sequence of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2DE). For each purified protein, a peptide mixture is generated by cleavage of the protein with cyanogen bromide. During incubation with carboxypeptidases only the original C-terminal fragment forms a ladder. Ladder readout is performed using MALDI mass spectrometry. 2DE-separated proteins from Shewanella oneidensis were chosen as a model system to investigate the effectiveness of the approach.".
- aggregation authorList BK1421994.
- aggregation endPage "482".
- aggregation startPage "469".
- aggregation volume "519".
- aggregation isDescribedBy 631538.
- aggregation similarTo 978-1-59745-281-6.
- aggregation similarTo LU-631538.