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- aggregation classification "A1".
- aggregation creator B314753.
- aggregation creator B314754.
- aggregation creator B314755.
- aggregation creator person.
- aggregation creator person.
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- aggregation date "2009".
- aggregation format "application/pdf".
- aggregation hasFormat 664840.bibtex.
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- aggregation hasFormat 664840.doc.
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- aggregation isPartOf urn:issn:0105-4538.
- aggregation language "eng".
- aggregation publisher "WILEY-BLACKWELL PUBLISHING, INC".
- aggregation subject "Science General".
- aggregation title "Differential expression of the interleukin 5 receptor alpha isoforms in blood and tissue eosinophils of nasal polyp patients".
- aggregation abstract "Given the key role of interleukin-5 (IL-5) in eosinophil function, we investigated the regulated expression of the membrane-anchored (TM-IL-5R alpha) isoform, or a secreted (SOL IL-5R alpha) isoform, on both protein and transcript level in vitro and in vivo. A real-time PCR, FACS and ELISA were established to determine IL-5R alpha isoform expression in peripheral blood and nasal tissue from control subjects and nasal polyp (NP) patients with or without asthma. Human peripheral blood eosinophils were incubated with IL-5 and were analyzed for SOL-IL-5R alpha and TM-IL-5R alpha mRNA and protein levels in comparison with CD-69 expression. SOL-IL-5R alpha and TM-IL-5R alpha mRNA and protein expression was significantly increased in NP vs controls. In polyp tissue, SOL-IL-5R alpha expression correlated to disease severity and eosinophils counts, whereas TM-IL-5R alpha levels were inversely correlated to eosinophils counts and SOL-IL-5R alpha expression. FACS analysis revealed increased CD-69 and decreased TM-IL-5R alpha expression in NP tissue eosinophils vs blood eosinophils. Incubation of blood eosinophils with IL-5 caused up-regulation of CD-69 and down-regulation of TM-IL-5R alpha after 2 and 24 h. The expression of SOL-IL-5R alpha and TM-IL-5R alpha differs according to the eosinophil activation state and localization in the body (blood vs tissue) and may therefore be involved in the fine-tuning of the eosinophil homeostasis. Exposure of eosinophils to IL-5 reduces their responsiveness to IL-5 by regulated expression of the IL-5R alpha isoforms. Since, TM-IL-5R alpha is down-regulated and SOL-IL-5R alpha (antagonistic) is upregulated in NP tissue, our findings are important to understand the clinical trials with anti-IL-5 in humans.".
- aggregation authorList BK603881.
- aggregation endPage "732".
- aggregation issue "5".
- aggregation startPage "725".
- aggregation volume "64".
- aggregation aggregates 3112593.
- aggregation isDescribedBy 664840.
- aggregation similarTo j.1398-9995.2008.01885.x.
- aggregation similarTo LU-664840.